Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 6
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum BABT
Zia,Muhammad Anjum; Riaz,Ayesha; Rasul,Samreen; Abbas,Rao Zahid.
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; Antimicrobial; Aspergillus niger; Penicillium notatum.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600010
Imagem não selecionada

Imprime registro no formato completo
Imobilização de glicose oxidase para a síntese de ácidos aldônicos. Infoteca-e
VAZ JUNIOR, S.; RODRIGUES, D. de S.; GONCALVES, S. B.; ALMEIDA, J. R. M. de; CARVALHO, W.; PACHECO, T. F..
Os ácidos aldônicos — glicônico e xilônico — estão entre as moléculas de alto valor agregado que podem ser obtidas a partir de biomassa lignocelulósica e apresentam elevado potencial de aplicação comercial em diferentes campos. A síntese de ácidos aldônicos é possível por meio de diferentes processos, como: químico, eletroquímico, microbiano e enzimático. Porém, independentemente da opção escolhida, ainda existem vários obstáculos para que a produção em larga escala seja bem estabelecida. Entre esses obstáculos, está o desenvolvimento de um catalisador eficaz e estável para a síntese dos ácidos aldônicos a partir de hidrolisados de biomassa lignocelulósica, cuja composição apresenta, além dos monômeros de interesse (glicose e xilose), um grande número de...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Biocatalisador; Ácidos aldônicos; Biocatálise; Reator de leito fixo; Ácido glicônico; Ácido xilônico; Glicose oxidase; Catalisador; Green chemistry; Biocatalysis; Biomass; Glucose oxidase.
Ano: 2020 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1124141
Imagem não selecionada

Imprime registro no formato completo
Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors BJPS
Silva,Aline Ramos da; Tomotani,Ester Junko; Vitolo,Michele.
Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrose/conversion; Fructose; Gluconic acid; Invertase; Glucose oxidase; Catalase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200022
Imagem não selecionada

Imprime registro no formato completo
Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol BABT
Singh,Jagdish; Verma,Neelam.
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Glucose oxidase; Aqueous two phase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007
Imagem não selecionada

Imprime registro no formato completo
Production of rabbit antibodies against purified Glucose oxidase BABT
Zia,Muhammad Anjum; Ain,Qurat-ul; Iftikhar,Tehreema; Abbas,Rao Zahid; Rahman,Khalil-ur.
Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg -1 specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) resulted into 22.53 U mg-1 specific activity and 10.27 fold purification. This was applied to sephadex G-200 column for gel filtration chromatography. It was observed that enzyme achieved 59.37 U mg-1of specific activity with 27.08 fold purity and 64.36% recovery. Purified glucose oxidase was injected into rabbits through intravenous route, to raise the glucose oxidase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; A. niger; Antibodies; Rabbits.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100008
Imagem não selecionada

Imprime registro no formato completo
Screening of filamentous fungi for production of enzymes of biotechnological interest BJM
Guimarães,Luis Henrique S.; Peixoto-Nogueira,Simone C.; Michelin,Michele; Rizzatti,Ana Carolina S.; Sandrim,Valéria C.; Zanoelo,Fabiana F; Aquino,Ana Carla M.M.; Junior,Altino B.; Polizeli,Maria de Lourdes T. M..
Many enzymes produced by fungi have relevant biotechnological applications in several industrial areas. The purpose of this study was to collect and isolate filamentous fungi from soil and humus, plants and sugar cane bagasse of different regions of the São Paulo state. Forty isolates were examined for their ability to produce xylanase, glucose-oxidase, alkaline phosphatase, acid phosphatase, phytase, pectinase and amylase. Among these, twenty three isolates exhibited enzymatic potential. The xylanases produced by two of these isolates (Aspergillus caespitosus and A. phoenicis) showed good potential for pulp bleaching. Among seventeen isolates, at least three produced high levels of glucose-oxidase, being Rhizopus stolonifer and A. versicolor the best...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphatase; Xylanase; Glucose oxidase; Pectinase; Amylase; Rhizopus microsporus var. rhizopodiformis; Fungi.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400014
Registros recuperados: 6
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional